Legends of Microbiology and Infectious Diseases - Robert Koch

The German physician Robert Koch (1843-1910)tuberculosis bacterium in 1882 and his studies in
contributed so much to the field of microbiology andtuberculosis later resulted in his winning the Nobel
infectious diseases. A contemporary of Louis Pasteur,Prize in Physiology or Medicine in 1905.
Koch worked as a physician most of his life.He is also recognized for his work in Africa with
Some of the great contributions to the field ofTrypanosoma and tick borne spirochetes.
microbiology and infectious diseases include identifyingBut what many think his greatest contribution in
the bacterium that causes anthrax (Bacillus anthracis)microbiology and infectious diseases is his formulation
in 1877. He recognized both the actively dividing cellsof four postulates that associate a particular
and the dormant cells (spores) and developedorganism with a specific disease.
techniques for studying them outside the body.Koch's postulates are as following:
Another great contribution, which microbiologists1. The specific causative agent must be found in
today can be thankful for, is developing a way toevery case of the disease.
grow bacteria in pure culture. After trying different2. The disease organism must be isolated in pure
media (potato slices, gelatin, etc) he used a cookingculture.
thickener (agar) which created a firm surface so the3. Inoculation of a sample of the culture into a
bacteria could be spread very thinly over the surface.healthy, susceptible animal must produce the same
The micro organism is spread so thinly that individualdisease.
organisms are separated from each other. Then after4. The disease organism must be recovered from the
time, each individual organism would multiply to forminoculated animal.
a visible colony from its millions of descendents.Implied in Koch's postulates are the one organism-one
Koch's discovery of the method of preparing puredisease concept and it provided the method of
culture of bacteria allowed German microbiologists toestablishing the germ theory of disease.
advance well ahead of microbiologists of France andRobert Koch had many other accomplishments in the
other nations who were still using broth culture andfield of microbiology and infectious diseases and was
successive dilutions to get a single organism.well respected on the continents of Africa and Asia
Other work attributed to Koch includes thefor his work.
discoveries of Vibrio cholera in 1883 and theHe died on May 27, 1910 from a heart attack.